The puppies should all be held and touched each day. This is very important part of socialization and touch desensitization. A puppy should become used to the human touch, human noises, being washed down with a warm soft washcloth, being handled and having his mouth, teeth and paws touched. This allows that puppy to then go on to be a happy, well socialized family member in its new home.
In addition, gradually, a newborn should be exposed to regular household noises such as the TV on at a normal volume...the radio, the dishwasher....noises that one would expect in any home. In this way, he or she will remain calm and not be startled throughout the day.
The 2nd de-worming may be done now. As with the 1st, always wait for your vet to approve this, as the exact time to do this will be determined by the pup's weight, health and progress.